Example Data

FCS list mode data is provided at the Cira website near the link where you've downloaded your copy of QTube.  There are two samples:  samp1 has a tube that probably had a partial clog, and samp2 is a highly consistent data set.  By comparing QTube's results for these data sets you can begin to get a feel for what you might look for in your data.

Important note:  the protocol for this Leukemia panel called for permeablization of tubes 7-10.  Thus, you should compare tubes 1-6 and tubes 7-10 in two separate QTube runs.

SAMP1

If you run samp1, tubes 1-6 in QTube looking at CD45 and SS, with default parameters, you should see the following:



Using your favorite Flow software, examine the dotplots.  You should note, as shown below, that Tube #6 is shifted a bit towards the origin.  Perhaps there was a partial clog for this tube.



SAMP2

If you run samp2, tubes 1-6 in QTube looking at CD45 and SS, with default parameters, you should see the following:



Again, examining the dotplots, you'll see that the distributions for these tubes is much more consistent with the contours drawn for the aggregate data.  Thus, this sample receives "green" scores.