FCS list mode data is provided at the Cira website near the link where
you've downloaded your copy of QTube. There are two samples:
samp1 has a tube that probably had a partial clog, and samp2 is a
highly consistent data set. By comparing QTube's results for
these data sets you can begin to get a feel for what you might look for
in your data.
the protocol for this Leukemia panel called for permeablization
of tubes 7-10. Thus, you should compare tubes 1-6 and tubes 7-10
in two separate QTube runs.
If you run samp1, tubes 1-6 in QTube looking at CD45 and SS, with default parameters, you should see the following:
Using your favorite Flow software, examine the dotplots. You
should note, as shown below, that Tube #6 is shifted a bit towards the
origin. Perhaps there was a partial clog for this tube.
If you run samp2, tubes 1-6 in QTube looking at CD45 and SS, with default parameters, you should see the following:
Again, examining the dotplots, you'll see that the distributions for
these tubes is much more consistent with the contours drawn for the
aggregate data. Thus, this sample receives "green" scores.