Problems and Solutions
Problem: After selecting a group of tubes, no parameters are available to be selected for fingerprinting.
Solution: Check the
parameters carefully for each tube (you may find it helpful to look at
File->Properties - this will list the parameters, along with
the total number of events, in each tube). If you've included
negative control tubes inadvertently, its $PnS values won't match
the other tubes, and QTube will (correctly) not allow the
analysis. If this is the case, re-select tubes, omitting the
Problem: I click on the Run button but nothing happens.
Solution: Have you selected parameters? You need to enable one or more of the common parameters in order for QTube to work.
Problem: Many or all of the metrics are RED. What does that mean?
Solution: There are at least three possibilities:
(1) You are using a resolution that's too high, given the number of parameters in play. Try reducing resolution (see Interpreting Results
for a detailed explanation).
(2) If you are already using "low" resolution, then these tubes are may
be from different samples (i.e different patients) and so the
distributions are very different. Remember, QTube is a QC tool to
make sure that multiple tubes from a single sample
self-consistent. Including multiple samples will generally yield
confusing (and probably meaningless) results.
(3) Even though your tubes all share a subset of common parameters
(often used to gate an entire panel), there may be a reason why these
distributions differ. We've seen for example in some Leukemia
panels that some of the tubes have been permeablized. This
significantly effects the distribution of events in the FSC, SSC and
CD45 gating parameters. In such a case it may make sense to QC
the the permeablized and non-permeablized tubes in two separate QTube